Analysis Of Proteins Extracted From The Leaves Of Piper Sarmentosum Roxb
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Date
2010-10
Authors
Shim, Siang yian
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Publisher
Universiti Sains Malaysia
Abstract
A gel based proteomic method has been developed for the leaf tissues of P. sarmentosum. A suitable protein extraction method termed as total protein extraction followed by trichloroacetic acid (TCA)/acetone precipitation was applied and has been shown to produce good leaf tissue extract that was compatible with 2-DE separation. The protein profiling of leaf tissues of P. sarmentosum showed a total of 242 protein spots and from which 283 proteins were identified by LCMS/MS analysis. Most of these identified proteins were involved in metabolism (including photosynthesis), protein destination and storage, energy and transport, and defense and stress related protein. In the comparative proteomic analysis on young and mature leaf tissues of P. sarmentosum obtained from seven geographical areas, 84 protein spots were found common in all the leaf extracts of P. sarmentosum regardless of young or mature leaf or geographical areas. Among these 84 common protein spots, 21 protein spots respectively in the young and mature leaves of P. sarmentosum were detected as differentially expressed proteins with a 2-fold change in intensity between the highest intense spot and the least intense spot when comparing between the seven geographical areas. In addition, 4 protein spots were detected as unique spots and 21 protein spots were detected as irregular protein spots. The proteome of leaf tissues of P. sarmentosum has been successfully mapped which revealed the protein composition of the leaf tissue of P. sarmentosum, a traditionally known leaf-based medicinal plant.
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Keywords
A gel based proteomic method has been developed , for the leaf tissues of P. sarmentosum