Haemoglobin (Hb) Loaded Water Soluble Hyperbranched Polymer For The Development Of Artificial Oxygen Carrier
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Date
2019
Authors
Ooi, Hui Wen
Journal Title
Journal ISSN
Volume Title
Publisher
Universiti Sains Malaysia
Abstract
The difficulties regarding demand, supply and storing of blood for therapeutic use
transfusion have initiated extensive efforts to develop artificial blood especially red cell
substitutes. The history of these hard work includes a complicated mixture business,
ethics and scientific field. As time passes towards expiry, RBC membrane loses its
structure integrity but the hB molecule remains intact. Free stroma hB is toxic as it can
cause kidney toxicity therefore chemical modification is needed for the continuity of hB
use. This study involves experimentation using hyperbranched polyglycidol to
encapsulate bovine hB and human hB extracted from expired blood donation to produce
red cell mimic. Polyglycidol is a hyperbranched polymer possessing around 50% to 60%
of dendrimeric structure. The main objective of this study is to study nano-encapsulation
of hB from expired blood human blood and bovine for development of red cell substitute
by isolation of human hB from expired blood from volunteer donors, encapsulation of
human and bovine hB in hyperbranched polymer called glycidol, and to evaluate
reversible oxygen binding of encapsulated human and bovine hB. Findings showed no
significant decrease of concentration (p > 0.05) between free-hB and encapsulated
complex for both hBs (p = 0.109). These demonstrates high encapsulation efficiency with
88.1% and 99.0% indicating least three hB molecules were entrapped within interior
chamber of the polyglycidol. For reversible oxygen cycles although average of five
reversible cycles was not significant (p > 0.05) for both encapsulated human hB (p =
0.109) and bovine hB (p = 0.109), addition of polyglycidol for encapsulation of the hBs prolonged the persistence of reversible oxygen cycle as compared to only one cycle
recorded by the free-hB prior to its complete deactivation. The comparison of
encapsulation efficiency (p = 0.100) and average of reversible oxygen cycles (p = 0.109)
between encapsulated human hB and encapsulated bovine hB were not significant (p >
0.05) demonstrating that the performance of bovine hB was similar behaviour to human
hB encapsulating and encapsulated in polyglycidol. These suggest that human hB could
also be substituted by bovine hB as the latter is easier to obtain. The utilisation of hBpolyglycidol
system signifies a noteworthy footstep in the progress of red cell substitute
production with extended oxygen delivery in the circulation.
Description
Keywords
Hemoglobins , Blood Substitues