Population Structure Based On Mitochondrial Cytochrome Oxidase Subunit I (Coi) Gene In The Black Scar Oyster, Crassostrea Iredalei (Faustino, 1932) In Malaysia
Loading...
Date
2013-12
Authors
Zainal Abidin, Danial Hariz
Journal Title
Journal ISSN
Volume Title
Publisher
Universiti Sains Malaysia
Abstract
Mitochondrial cytochrome oxidase subunit I (COI) gene was utilised to
assess the population genetics and phylogeographic relationships of the
commercially important black scar oyster, C. iredalei among 11 populations
throughout Peninsular Malaysia and Sabah. Overall, populations of C. iredalei
demonstrated low nucleotide diversity π (0.000 – 0.004) and low-to-high haplotype
diversity h (0.000 – 0.795) levels. Genetic structuring was detected between the
Peninsular Malaysia and Sabah populations as revealed by the FST and AMOVA
analyses. However, the COI gene analyses showed minimal and non-significant
(P>0.05) population differentiation within the Peninsular Malaysia and Sabah
regions. Relative genetic homogeneity was reported within the coastal populations of
Peninsular Malaysia and Sabah, respectively. These could be attributed to both high
larval dispersal along the east and west coasts and human-driven spat translocation
between the two coastlines due to C. iredalei cultivation practices. The neutrality and
mismatch distribution analyses supported the hypothesis that C. iredalei had
experienced a/several bottleneck event(s), followed by population expansion. The
molecular information obtained from this study can be incorporated in a pragmatic
aquaculture management strategy of wild broodstock and the hatchery lines of C.
iredalei in Malaysia. A Next Generation Sequencing (NGS) approach, Ion Torrent
technology was utilized in the development of novel microsatellite markers in C.
iredalei. Although a total number of 2424 microsatellite motif repeats were
identified, of which, 137 qualified for primer design in the present study.
Description
Keywords
Mitochondrial Cytochrome Oxidase Subunit I (Coi) , Black Scar Oyster