Development and validation of HPLC method for simultaneous determination of haloperidol and reduced haloperidol in plasma
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Date
2006
Authors
Asualingam, Theepa
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Abstract
Haloperidol is a typical antipsychotic drug that chemically belongs to
butyrophenone group. A sensitive and selective reversed-phase HPLC method with
ultraviolet detection was developed for the simultaneous determination of haloperidol
and reduced haloperidol in plasma. Drugs were detected at 230 nm. The
chromatographic separation was performed on an Inertsil C8-3 (150 x 4.6mm, 5 μm)
HPLC column. Mobile phase composed of 50 mM phosphate buffer pH 5.0 and
methanol (51:49, v/v) and eluted at 1.0 ml/min. A simple liquid-liquid extraction was
carried-out using pyrimethamine as an internal standard. The mean extraction recovery
for haloperidol, reduced haloperidol and pyrimethamine were 82.4, 82.1 and 82.0%
respectively. The method showed good selectivity with respect to commonly
administered psychotropic drugs. Calibration curve was linear over the concentration
range 1 - 60 ng/ml with the correlation coefficient (r) > 0.999. The within-day and day-to
-day assay precision was less than 5%. Limit of detection and lower limit of
quantification were 0.5 ng/ml and 1 ng/ml respectively for haloperidol and reduced
haloperidol.
Stock standard of haloperidol and reduced haloperidol in methanol were
stable up to three months at -20°C. Plasma samples spiked with haloperidol and
reduced haloperidol was stable up to 6 months at -20°C and -85°C. The method was
successfully applied to pharmacokinetic study of haloperidol in rats following oral dose
of haloperidol (2.5 mg/kg). The mean t1/2, Cmax, tmax, AUC (0-∞), CL and Vd were 6.2 ± 2.6
h, 24.9 ± 7.2 ng/ml, 2.0 ± 1.9 h, 214.1 ± 76.0 ng.h/ml, 213.0 ± 61.9 ml/min/kg and
110.3 ± 46.3 l/kg respectively. The analytical method for haloperidol and reduced
haloperidol assay is found to be suitable for pharmacokinetic studies.
Description
Master
Keywords
Pharmaceutical science , HPLC method , Haloperidol , Pharmacokinetic