Some Pharmacological And Phytochemical Studies Of Clitoria Ternatea Extracts

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dc.contributor.authorLinggam, Kamilla
dc.date.accessioned2020-02-06T00:48:41Z
dc.date.accessioned2020-02-06T00:48:44Z
dc.date.available2020-02-06T00:48:41Z
dc.date.available2020-02-06T00:48:44Z
dc.date.issued2012-01
dc.description.abstractIn this thesis, detailed work was carried out to explore and determine the phytochemical screening, potential anti-infective, antioxidant and analgesic properties of C. ternatea plant parts using various bioassays techniques and appropriate experimental models. The initial qualitative phytochemical screening shows that C. ternatea plant parts contain tannin, phlobatannin, flavonoid, anthraquinone, alkaloid, saponin, cardiac glycosides, volatile oils, steroids and terpenoids. The flavonoids and phenolic content of the various plant parts were also determined in the plant parts. Analysis shows that C. ternatea flower extract has both high phenolic (104.77 ± 2.64 mg GAE/g) and flavonoid (28.10 ± 4.13 mg CE/g) content than other plant parts. This is evident by the fact that C. ternatea flower extract exhibited the highest antioxidant activity (FRAP: 6.80 ± 0.32 μM Fe(II)/g ; DPPH IC50 : 9.17 ± 0.003 μg/mL) when compared to other parts of the plant. One of the bioactives identified in the flower extract was kaempferol-3-glucoside (K3G) which is a flavonoid compound. Kaempferol-3-glucoside was also found in C. ternatea stem and leaf. This could be one of the identified bioactives responsible for the antioxidant activity. As for the in vitro anti-infective screening, both leaf and root crude extracts demonstrated marked antimicrobial activities. Interestingly, the leaf extract demonstrated a remarkable antifungal activity against Aspergillus niger with a MIC and MFC of 0.4 and 0.8 mg/mL, respectively. This was evident by the scanning electron microscope (SEM) analysis which showed a marked morphological alteration of the A. niger hyphal wall and conidiophores after exposure to C. ternatea leaf extract when compared to control. The acute toxicity of C. ternatea leaf extract was assessed in mice prior to antinociceptive and in vivo antifungal study. After oral administration in mice, the LD50 was found to be higher than 2000 mg/kg body weight which indicated some high level of safety for C. ternatea leaf extract. Subsequently C. ternatea leaf extract was further tested for its antinociceptive and in vivo antifungal activities in animal models. Studies in rats showed that both C. ternatea leaf and root extracts demonstrated antinociceptive activity at the studied dosage regimens when compared with control but the analgesic effect was not comparable to morphine treated rats. In another study, the in vivo antifungal activity of C. ternatea leaf extract was investigated in A. niger infected mice. C. ternatea leaf extract antifungal activity was comparable to clinically used amphotericin B. Bioactivity guided fractionation studies of the C. ternatea leaf extract suggest that the antifungal activity could be a synergistic activity of various bioactives presence in the extract. Further gas chromatography-mass spectroscopy (GC-MS) and fourier transform infrared (FTIR) analysis of the fractions indicated these bioactives could be lipids and/or terpenoids (sesquiterpenes) group of compounds.en_US
dc.identifier.urihttp://hdl.handle.net/123456789/9502
dc.language.isoenen_US
dc.subjectCentrosemaen_US
dc.titleSome Pharmacological And Phytochemical Studies Of Clitoria Ternatea Extractsen_US
dc.typeThesisen_US
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