Effects Of Selective Dietary Prebiotics And Probiotics On Growth And Health Status Of Snakehead (Channa striata) Fingerlings
Loading...
Date
2016-08
Authors
Munir, Mohammad Bodrul
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
Intensive culture of snakehead has resulted in problems such as deterioration of water quality and the outbreak of diseases. Currently, fish disease is managed through the use of antibiotics which has led to antimicrobial resistant pathogens, reduction in beneficial microbiota in the gastrointestinal (GI) ecosystem, including the accumulation of residual antibiotics in fish muscle making it unsuitable for human consumption. To overcome these problems a new feeding approach using prebiotics and probiotics is explored. The present research was conducted to evaluate the effect of feeding Channa striata fingerlings with different prebiotics and probiotics as well as duration of feeding on growth and health performance. The experimental design involved feeding experimental fish with β-glucan, Galacto-oligosaccharides (GOS), Mannan-oligosaccharides (MOS), live yeast (Saccharomyces cerevisiae) and LBA (Lactobacillus acidophilus) powder respectively, for a total of 16 weeks (Phase 1) followed by feeding of a control unsupplemented feed for 8 weeks (Phase 2). Duplicate groups of 800 fish (22.40 g+ 0.06) were raised on six different treatments respectively, three prebiotics - 0.2% β-glucan, 1% GOS, 0.5% MOS, and two probiotics - 1% live yeast, 0.01% LBA and a control (unsupplemented) diet. All diets contained 40% protein and 12% lipid. Fish were fed to satiation three times daily. After 16 weeks of feeding, prebiotics and probiotics supplemented diets improved growth performance, relative protein digestibility, digestive enzymes activities, gut microflora, haematological and
immunological blood parameters, disease resistance against Aeromonas hydrophila and the expression of immune regulatory genes significantly (P<0.05) compared to the control diet. Among the supplemented diets feeding with probiotics resulted in better performance compared to the three prebiotics tested, with highest performance in fish fed with L. acidophilus. Although fish fed the β-glucan supplemented diet showed better performance for all the parameters monitored after 8 weeks of feeding compared to GOS and MOS supplemented diets, no significant differences were observed by the 16th week of feeding. In Phase 2, fish growth continued until the 5th and 6th week, for S. cerevisiae and L. acidophilus, respectively and up to 4 weeks for the prebiotics supplemented diets before decreasing. The results of gut microflora analysis using T-RFLP method revealed that bacterial community richness and evenness were enhanced regardless of dietary supplements compared to the control diet (38) while LBA resulting in the highest number (49) of bacterial phylotypes. The expression of immune regulatory two genes (TGF β1 and NF кB) were up-regulated in all supplemented diets. The results obtained from the present study showed that supplementation with L. acidophilus significantly (P<0.05) supports not only best growth and health performance of C. striata fingerlings but this advantage is retained over a longer period compared to feeding with diets containing S. cerevisiae and the other prebiotics.
Description
Keywords
The effect of feeding Channa striata fingerlings with different prebiotics and probiotics , of feeding on growth and health performance.