Molecular characterization of Selar crumeenophthabnus (ikan Lolong), Decapterus 111aruadsi (ikan Sadin) and Rastrelliger kanagurta (ikan kembong hitatn) fish species using mtDNA COI gene (DNA barcoding)
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Date
2011
Authors
Kee, Loo Suet
Journal Title
Journal ISSN
Volume Title
Publisher
Pusat Pengajian Sains Kesihatan, Pusat Pengajian Sains Perubatan
Abstract
This study was undertaken to design a set of common primers to amplify the mitochondria
DNA (mtDNA) cytochrome coxidase subunit I (COl) barcoding region of marine fish species.
The primer set namely: forward primer (ST F I) "CAC AAA GAC ATC GGC ACCC" and the
reverse primer (ST R3) "AAG AAT CANG AAT AG/AG TOT TG" were able to amplify the
three species chosen for this study. The sequence data generated were compared and analysed
using the sequence of mtDNA COl gene of Gobio gobio (GenBank accession no.: AB239596)
as reference. On analysis, about 20% polymorphic positional variations were detected across
the three species. The sequence data generated were used to study their phylogenetic
relationship by constructing a rooted neighbor joining (NJ) dendrogram
Description
Keywords
mitochondria DNA