Effect Of Sandoricum Koetjape (Buah Sentul) Extracts On Hypocholesterolemic Activity Using 3- Hydroxy-3-Methylglutaryl-Coenzyme A Reductase (Hmgr) Assay
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Date
2013-03
Authors
Yeong, Ai Lin
Journal Title
Journal ISSN
Volume Title
Publisher
Universiti Sains Malaysia
Abstract
Sandoricum koetjape, a tropical fruit tree rich in phytochemical terpenoids,
was postulated to suppress cholesterol synthesis through inhibition of HMG-CoA
reductase (HMGR) activity. HMGR, a rate-limiting enzyme of cholesterol synthesis
consists of two domains, N-terminal sterol-sensing domain (residues 1-339) and Cterminal
catalytic domain (residues 460-888). This research was designed to study
the hypocholesterolemic activity of S. koetjape extract through inhibition of
recombinant human HMGR. In this study, the gene fragment encoding the catalytic
domain of HMGR was expressed and purified as a recombinant glutathione Stransferase
tagged protein. In vitro enzymatic assay revealed catalytic activity based
on the kinetic measurements. Methanolic crude extracts of leaves, bark, fruit hull and
flesh from S. koetjape exhibit in vitro inhibition activity significantly on the
recombinant human HMGR. Bark was found to be the most potent inhibitor with
IC50 <5 μg/ml, followed by fruit hull (IC50 = 32.5 μg/ml), leaves (IC50 = 37.6 μg/ml)
and flesh (IC50 = 68.3 μg/ml). In summary, all four plant parts of S. koetjape
exhibited hypocholesterolemic activity through HMGR inhibition. The promising
finding revealed that S. koetjape is a good candidate for further research as a
hypocholesterolemic agent.
Description
Keywords
Sandoricum Koetjape (Buah Sentul) Extracts , 3- Hydroxy-3-Methylglutaryl-Coenzyme A Reductase (Hmgr) Assay