Effect Of Ocimum Sanctum (Tulsi) Leaf Extract On Prothrombin Time (PT), Activated Partial Thromboplastin Time (APTT) And Thrombin Time (TT) In Human Plasma
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Date
2016
Authors
Gunendren, Miraashini
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Journal ISSN
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Publisher
Universiti Sains Malaysia
Abstract
Even tough, the conventional anticoagulant therapy proven to be effective in deep vein thrombosis and pulmonary embolism but there are several drawbacks such as bruises, pain, swelling or coughing blood. Therefore, the search for novel anticoagulant derived from natural substances like plants are demanded nowadays. Ocimum sanctum also known as Ocimum tenuiform (OT), tulsi or holy basil from the family of Lamiaceae has been widely used for thousands of years in Ayurveda and Unani system to cure or prevent a number of illness such as headache, malaria fever, ulcers, bronchitis, cough, flu, sore throat and asthma. The aim of this study is to investigate the effect of Ocimum sanctum (Tulsi) leaf extract on prothrombin time (PT), activated partial thromboplastin time (APTT) and thrombin time (TT) in human plasma. The coagulation activity of O.sanctum was measure via PT, APTT and TT assay in citrated plasma collected from thirty-six healthy regular blood donors. The plasma was tested against different concentration of O.sanctum aqueous extract as follows 0.1mg/ml, 0.5mg/ml and 1.0mg/ml. The result showed, the aqueous extract of O.sanctum prolonged the PT and APTT assays (p<0.05) but no affect on TT assay (p>0.05). The GCMS analysis had identified the linolenic acid at 1-10% of ethanol and aqueous concentration at different retention time which was responsible for the coagulation activities of O.sanctum in human plasma. In conclusion, this study suggests that O.sanctum does affect coagulation activity in human plasma and can be potentially used as naturally derived anticoagulant products in future.
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Keywords
Effect Of Ocimum Sanctum (Tulsi) Leaf Extract , On Prothrombin Time