Molecular Identification, Geometric Morphometrics And Phylogenetic Relationship Of Commercially Important Nemipteridae From Malaysian Waters And Neighboring Seas Inferred By Mtdna And Nuclear Genes

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dc.contributor.authorImtiaz, Ayesha
dc.date.accessioned2019-01-24T02:16:58Z
dc.date.available2019-01-24T02:16:58Z
dc.date.issued2018-01
dc.description.abstractThis study employed genetic and morphometric approaches to assess the molecular and morphometric differentiation as well as phylogenetic relationships among commercially important species of family Nemipteridae. Molecular investigations were conducted based on two mitochondrial and one nuclear genes on 210 individuals within 13 presumed species of family Nemipteridae from seven marine regions; Indian Ocean, Straits of Malacca, East South China Sea (ESCS), West South China Sea (WSCS), North South China Sea (NSCS), Sulu Sea (SS) and Celebes Sea (CS). In the first part of the study, the DNA barcoding method was employed using the standard region of barcoding mitochondrial cytochrome oxidase c (COI) (647 bp) to identify and potentially discovered new species. All samples were statistically analysed in MEGA v7.0 and clustered into fourteen respective putative species. Intraspecific genetic distance of > 2% suggested the potential occurrence of cryptic species within the presumed N. japonicus and S. vosmeri individuals. An average of 10X differences was observed between mean inter and intra specific genetic distance among all taxa except N. japonicus and S. vosmeri. In the next part of this study, quantitative data based on geometric morphometrics technique of 19 homologous landmarks were analyzed on 150 individuals within 13 presumed species with Multivariate Analysis of Variance (MANOVA) in various tps programs and Morpho J software. Principal component analysis (PCA) generated 34 components with very low eigenvalue of < 1 and thus of negligible significance. However, Canonical Variate analysis (CVA) generated 12 canonical variates, nine had eigenvalue of >1 with the highest eigenvalue from CV1 that explained 76% of body shape variations and could efficiently discriminate among the 13 taxa analysed.en_US
dc.identifier.urihttp://hdl.handle.net/123456789/7640
dc.language.isoenen_US
dc.publisherUniversiti Sains Malaysiaen_US
dc.subjectGenetic and morphometric approaches to assessen_US
dc.subjectmolecular and morphometric differentiation as well as phylogeneticen_US
dc.titleMolecular Identification, Geometric Morphometrics And Phylogenetic Relationship Of Commercially Important Nemipteridae From Malaysian Waters And Neighboring Seas Inferred By Mtdna And Nuclear Genesen_US
dc.typeThesisen_US
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