PSYCHOPHARMACOLOGY OF Centella asiatica : IN VIVO AND IN VITRO STUDY
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Date
2012-06
Authors
Mohd Nasir, Mat Nor
Journal Title
Journal ISSN
Volume Title
Publisher
Pusat Pengajian Sains Perubatan Universiti Sains Malaysia
Abstract
Centella asiatica has been identified to reduce the declining cognitive function
in studies done by ethnopharmacological scientists. In this study, asiatic acid from
triterpenoid groups isolated from C. asiatica was examined for in vitro thin layer
chromatography (TLC) bioautographic acetylcholinesterase inhibitory enzyme activity,
in vivo psycopharmacology of excitatory post synaptic potential (EPSP) on the rat
hippocampus and in vivo learning and memory potential. The mechanisms of the blood
pressure with locomotors performances were also performed. Asiatic acid showed an
effect on TLC bioautographic acetylcholinesterase inhibitory activity with minimal
inhibition concentration which was 0.125 μg. Asiatic acid had selectively reacted to
gamma aminobutyric acid receptor B (GABA B) agonist for in vivo psycopharmacology
of EPSP. Then the inhibitory concentration of GABA B was plotted with half maximal
inhibitory concentration, of 14 μM and Hill-Coefficient 0.9828. The in vivo learning
and memory potential were performed using passive and active avoidance devices.
Administration of 30 mg/kg asiatic acid facilitated the passive avoidance on memory
but had no effect on learning in active avoidance. Study on the effect of blood pressure
with locomotors performances showed that 10 and 30 mg/kg asiatic acid reduced blood
pressure and there was no sedative effect on locomotor performance.
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Keywords
Neuroscience