The establishment of isothermal amplification and lateral flow device for detection of salmonella typhi and salmonella paratyphi A

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dc.contributor.authorHanaphi, Roziana Mohamed
dc.date.accessioned2021-04-14T06:11:22Z
dc.date.available2021-04-14T06:11:22Z
dc.date.issued2020-11
dc.description.abstractspecificity for both targeted bacteria detection on DNA of bacteria isolates of 30 S. Typhi, 30 S. Paratyphi A, 30 Salmonella serovars and 30 non-Salmonella bacteria. The limit of detection of the CPA for S. Typhi assay was 1 fg of genomic templates and 101 CFU/ml in bacteria culture, while, the CPA for S. Paratyphi A assay was 10 fg of genomic templates and 103 CFU/ml in bacteria culture. The results obtained were consistent for both S. Typhi and S. Paratyphi A assays for the detection method with agarose gel electrophoresis (AGE) and LFA. The sensitivity and specificity of both assays were effective when tested with 54 spiked-stool samples, 56 water samples from endemic areas and three stool samples of suspected carriers. The final form of lyophilised CPA reagents was obtained provided ready-to-use reagents together with lateral flow strips for limited resource settings and transportation without a cold chain. The stability testing of both assays in different storage temperatures of 4°C, 25°C, 37°C and 45°C revealed the shelf-life of three to four months when tested in 30 days storage. As a conclusion, the CPA was effective in the detection of S. Typhi and S. Paratyphi A, which was ideal for limited-resource settings with rapid detection, simple procedure and equipment, and easy readout of the assay results using LFA.en_US
dc.identifier.urihttp://hdl.handle.net/123456789/12809
dc.language.isoenen_US
dc.publisherPusat Pengajian Sains Perubatan, Universiti Sains Malaysiaen_US
dc.subjectSalmonella infectionsen_US
dc.titleThe establishment of isothermal amplification and lateral flow device for detection of salmonella typhi and salmonella paratyphi Aen_US
dc.typeThesisen_US
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