Investigation Of Chemopreventive Activity With Two Different Varieties Of Freeze Dried Coconut Water (Fdcw) On Cervical Cancer Cell Line
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Date
2015-07
Authors
Ramlee, Siti Nur Liyana
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Journal ISSN
Volume Title
Publisher
Universiti Sains Malaysia
Abstract
Coconut water is considered as nature’s elixir to mankind. Not only as a refreshing beverage to quench the thirst, coconut water is consumed starting from infancy to adulthood as a health tonic especially by people of the tropics. With increasing trend of cancer prevalence, novel anticancer therapies such as chemoprevention approach urgently needed. In this study, it was found that coconut water exert its anti-proliferative activity against HeLa cells with IC50 value of 100 μg/ml in both MATAG FDCW and Aromatic Dwarf (AD) FDCW varieties. After 72 hours of treatment, observation under inverted microscope showed typical apoptotic morphological alteration in HeLa cells exposed with MATAG FDCW. Meanwhile, features of apoptosis and autophagy observed in HeLa cells treated with AD FDCW. Observation under fluorescence microscope showed chromatin condensed and apoptotic bodies in both treatments against stained HeLa cells with Hoechst dye. To evaluate the anti-proliferative activity with prolong treatment exposure, treated HeLa cells with respective treatments were subjected to eight days of incubation period. Results showed that MATAG FDCW was able to continuously suppressed HeLa cells proliferation until final day of treatment. However, AD FDCW effect was instable with prolonged time hence, the suppression effect was reduced. Thus, the results suggested that MATAG FDCW exerts better anti-proliferative effect compared to AD FDCW. In conclusion, both FDCW varieties demonstrated positive chemopreventive activity thence considered as potential novel anticancer therapies.
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Keywords
Chemoprevention