Effects Of Two Different Maturity Of Freeze Dried Coconut Water On Cervical Cancer Chemoprevention Using In Vitro Analysis
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Date
2015
Authors
Kamaruzaman, Amirul Amin
Journal Title
Journal ISSN
Volume Title
Publisher
Universiti Sains Malaysia
Abstract
With the increasing concerns about chemoprevention in Malaysia, the utilizing of natural
and waste from botanical product might have beneficial effects towards cancer
chemoprevention study. Chemoprevention refers to the efforts to prevent and delay the
development of cancer by using natural or synthetic agents. In Malaysia, coconut is the
fourth important industrial crop after oil palm, rubber and paddy. The major variety
grown is the Malayan Tall, MATAG, MAWA, PANDAN and Malayan Dwarfs. This
study involves comparative chemoprevention effect between MATAG young which is 5
to 6 months age and MATAG Old which is 6 to 9 month age using In Vitro analysis on
cervical cancer cell line (HeLa). The IC50 of both maturities on HeLa cells were
determined with Trypan Blue Exclusion Assay (TBEA) method with an IC50 of
100μg/mL. Cell proliferation assay was monitored and showed an effective inhibitory
effect on HeLa cells. The inhibitory concentration on dose exposure of Cocus nucifera
shows that there are significant different in MATAG young in reduces the viability of
HeLa cells in a concentration and time dependent manner compared to MATAG old. It
shows that there are mean different in cell viability of each dose in MATAG young
p<0.001 while there are no different in MATAG old p>0.05. Microscopic observation of
cell proliferation assay and Hoescht 33342 stain shows that Cocus nucifera treatment
induces apoptotic features of cells. This is a preliminary study of coconut water on cancer
cell line and further analysis of the cell proportion on molecular levels need to be
continue in future. It was concluded that the MATAG young extracts exhibited a better
chemoprevention effect on HeLa cells.
Description
Keywords
Chemoprevention