Functional Characterization Of Styr-3 Non-Protein-Coding Rna (npcRNA) In Salmonella enterica serovar Typhi

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dc.contributor.authorKumar, Thiviyaa M. Othaya
dc.date.accessioned2019-07-17T01:39:04Z
dc.date.available2019-07-17T01:39:04Z
dc.date.issued2014-12
dc.description.abstractNon-protein-coding RNAs (npcRNAs) are RNA molecules that do not encode for proteins but are involved in modulating a myriad of cell regulatory processes, including bacterial drug resistance mechanisms. In this study, a novel npcRNA, StyR-3; discovered from the genome of S. Typhi was selected for further characterization via bioinformatics and experimental-based approaches. This 144-nt intergenic npcRNA is located in the ramRA regulon of S. Typhi Ty2. The bioinformatics-based characterization showed that it is highly conserved among Salmonella species; besides having a stable secondary structure as was predicted by LocaRNA software. According to BPROM software, StyR-3 was predicted to possess its own promoter. Furthermore, RNApredator; a target RNA search program showed that StyR-3 could form a stable interaction with the ramR mRNA, which further suggest its potential role in the regulation of MDR cascade. This was then followed by cloning and overexpression of StyR-3 in an S. Typhi clinical strain (ST001) in order to determine its effect upon the mRNA levels of ramA and ramR. The Northern blot expression result suggest that the overexpression of StyR-3 does not incur significant genotypic effect over the ramA and ramR mRNA levels. Moreover, disk diffusion assay that was carried out to determine the phenotypic effect of StyR-3 overexpression showed that both; the recombinant and the nonrecombinant strains were susceptible to 7 out of 8 drugs tested. Collectively, these results suggest that StyR-3 is most probably not involved in MDR regulation but it could possibly exert other biological roles.en_US
dc.identifier.urihttp://hdl.handle.net/123456789/8512
dc.language.isoenen_US
dc.publisherUniversiti Sains Malaysiaen_US
dc.subjectCharacterization of STYR-3 non-protein-coding RNAen_US
dc.subjectin Salmonella enterica serovar Typhien_US
dc.titleFunctional Characterization Of Styr-3 Non-Protein-Coding Rna (npcRNA) In Salmonella enterica serovar Typhien_US
dc.typeThesisen_US
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