Assessment Of Genetic Relatedness Among Hydrilla Verticillata (L. F.) Royle Accessions In Malaysia Using Rapd And Aflp Markers

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dc.contributor.authorChin, Choke Lin
dc.date.accessioned2018-05-31T06:36:09Z
dc.date.available2018-05-31T06:36:09Z
dc.date.issued2010-03
dc.description.abstractGenetic relationships among 119 accessions of Hydrilla verticillata from 28 locations in Malaysia were determined using Random Amplified Polymorphic DNA (RAPD) and 82 accessions of H. verticillata from 26 locations were studied using the Amplified Fragment Length Polymorphism (AFLP) technique. A total of nine RAPD primers produced 143 amplified fragments with 105 of them were polymorphic. RAPD data used to generate a Neighbour-Joining tree and Principal Coordinate Analysis (PCO) clustered these accessions into four groups that did not separate them according to geographical areas. A total of 326 polymorphic bands were generated using the AFLP technique with two primers combination. The Neighbour-Joining tree produced from the AFLP data divided the accessions into four groups that closely resemble those from RAPD analysis. However, the PCO results is dissimilar with Neighbour-Joining in which only three groups were recognized. Overall the level of genetic variation among accessions within a location is lower as compared to between accessions from different locations. This shows that it is important to understand the reproduction of the species within a location.en_US
dc.identifier.urihttp://hdl.handle.net/123456789/5613
dc.language.isoenen_US
dc.publisherUniversiti Sains Malaysiaen_US
dc.subjectGenetic relationships among 119 accessionsen_US
dc.subjectof Hydrilla verticillata from 28 locations in Malaysiaen_US
dc.titleAssessment Of Genetic Relatedness Among Hydrilla Verticillata (L. F.) Royle Accessions In Malaysia Using Rapd And Aflp Markersen_US
dc.typeThesisen_US
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