Porcine Dna Detection In Common Tablet Formulations And Excipients
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Date
2016-09
Authors
Syed Saberi, Syarifah Nur Syakira
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Abstract
Market demand on halal pharmaceutical products has increased due to the awareness of Muslim consumers towards halal products. With DNA verification technology, porcine content in adulterated excipients and final product can be detected by performing quantitative polymerase chain reaction (qPCR) test. In this study, tablet was chosen and three types of tablet formulation were selected; uncoated, film-coated and sustained-release tablets. Porcine DNA detection was assessed in each excipient used in formulating the three types of tablets. A template of positive and negative controls of uncoated tablet, film-coated tablet and sustained-release tablet were developed according to pharmaceutical standard for porcine DNA detection. Porcine and bovine gelatines were used as a spiking agent in each excipient and as a binder in positive and negative controls tablets. As a result, porcine DNA was detected in all spiked excipients and positive control tablets. It was found that CTAB solution was able to entrap DNA from polysaccharide matrix contained in sodium starch glycolate and pregelatinised starch. The results indicated that kaolin contained in coating excipients has a high affinity to DNA and in alkaline condition, the tight interaction of kaolin and DNA can be weakened. The results showed that porcine DNA was still able to be retrieved and detected after the drying process for uncoated tablet production. Thus, porcine DNA detection using qPCR testing method can be applied to detect porcine content in tablet and its excipients, when certain extraction techniques are adhered to.
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Porcine content in adulterated excipients and final product can be detected , by performing quantitative polymerase chain reaction (qPCR) test.