Production Of Tannase By A Local Strain Aspergillus Niger Fetl Ft3 In Submerged Culture System

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dc.contributor.authorGaneshan, Sumathi
dc.date.accessioned2019-10-03T07:25:16Z
dc.date.available2019-10-03T07:25:16Z
dc.date.issued2011-02
dc.description.abstractA tannase-producing fungal culture identified as Aspergillus niger FETL FT3 was isolated locally from hydrolysed tannin extract of Rhizophora apiculata barks. The potential of the fungal culture for enhanced extracellular production of tannase was improved in submerged fermentation (SmF) system. The improvement of various process parameters for maximum production of extracellular tannase from the fungal culture was carried out with free and immobilized cells in 250 ml shake flasks. Subsequently the improved cultivation conditions of shake flasks were transferred to a 2000 ml tubular air lift bioreactor and some selected variables were further improved. In shake flasks, maximum extracellular tannase production of 2.81 U/ml was obtained after 4 days of fermentation at 30oC with 1% (v/v) of 1 × 106 spores/ml inoculum size, initial medium pH 6.0 and agitation at 200 rpm in 50 ml of modified Czapek Dox minimal medium containing 3 wt%-C tannic acid, 0.8 wt%-C glucose and 0.048 wt%-N sodium nitrate in the case of free cells. The immobilized cells on nylon scouring mesh produced maximum extracellular tannase of 3.98 U/ml, also after 4 days of fermentation via conditions similar to that of free cells using 6 pieces of nylon scouring mesh cubes. However, lower tannase activities of 2.14 U/ml and 3.12 U/ml respectively were obtained in the 2000 ml tubular air lift bioreactor containing 1800 ml of the fermentation medium for both free and immobilized Aspergillus niger FETL FT3 cells.en_US
dc.identifier.urihttp://hdl.handle.net/123456789/8930
dc.language.isoenen_US
dc.publisherUniversiti Sains Malaysiaen_US
dc.subjectTannaseen_US
dc.subjectAspergillus Niger Fetl Ft3en_US
dc.titleProduction Of Tannase By A Local Strain Aspergillus Niger Fetl Ft3 In Submerged Culture Systemen_US
dc.typeThesisen_US
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