Expression Of PhaC1 And PhaC2 From Pseudomonas Sp. USM4-55 In Escherichia Coli Expression System

Simple item page
dc.contributor.authorAb. Ghani, Balqis
dc.date.accessioned2022-06-29T02:47:25Z
dc.date.available2022-06-29T02:47:25Z
dc.date.issued2007-09
dc.description.abstractPolyhydroxyalkanoates (PHAs) are synthesized intracelullarly by a variety of microorganisms as reserve materials for carbon and energy source. PHA synthase (PhaC) is the key enzyme in the polymerization of PHAs. Pseudomonas sp. USM4-55 is a local isolate which is able to produce a blend of short chain length and medium chain length of polymer. Genes coding for PhaC (phaC1 and phaC2) from Pseudomonas sp. USM4-55 were amplified (each with a size of 1.7 kb) from the genomic DNA, cloned into pGEMR-T for sequencing and then expressed. For expression studies, pQE-30 was used as the expression vector under the control of PT5 promoter. These clones were named pQE-C1 and pQE-C2, and were induced with 0.01 mM of isopropyl-p-Dthiogalactoside (IPTG) at ODeoo of 0.6.en_US
dc.identifier.urihttp://hdl.handle.net/123456789/15506
dc.language.isoenen_US
dc.publisherUniversiti Sains malaysiaen_US
dc.subjectPhaC1 And PhaC2en_US
dc.subjectEscherichia Colien_US
dc.titleExpression Of PhaC1 And PhaC2 From Pseudomonas Sp. USM4-55 In Escherichia Coli Expression Systemen_US
dc.typeThesisen_US
Files
License bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Pusat Pengajian Sains Kajihayat - Tesis