Isolation And Characterization Of Streptomyces sp. Isolated From Unexplored Estuarin Mangrove Sediments For Antioxidants Activities
| dc.contributor.author | Lat, Farizah Hanim | |
| dc.date.accessioned | 2019-03-01T01:45:56Z | |
| dc.date.available | 2019-03-01T01:45:56Z | |
| dc.date.issued | 2018-05 | |
| dc.description.abstract | In this study, the potency of eighty actinomycetes, isolated from mangrove sediment in states Perak and Kedah of Malaysia, were evaluated for production of antimicrobial and antioxidant metabolites. Five Streptomyces sp. that demonstrated antimicrobial activity against pathogenic bacteria in a preliminary screening were cultivated in ISP1 (International Streptomyces Project 1) broth and extracted using ethyl acetate at a ratio of 1:1 (v/v). Five active isolates were titled as MBL 159, MBL 161, MBL 169, MBL 185 and MBL 201. Ethyl acetate extract of MBL169 with accession number (KU663071) expressed the highest antimicrobial activity on the disc diffusion method and minimum inhibitory concentration (MIC) test. Free radical scavenging assay revealed MBL201 (KU705719) exhibited the highest percentage of scavenging activity at 92.36±6.50 percent, total antioxidant capacity (80.03±7.63 μg AAE/ml), ferric reducing (0.274±0.007 OD) and β-carotene-linoleate bleaching (zone of color retention: 3.25±0.16 mm) assays. The newly isolated Streptomyces species were identified using morphological characterization and 16S rRNA gene sequence analysis. The phylogenetic tree was constructed to perceive the isolates in close affinity to the Streptomyces species. The outcomes of this study specify that the ethyl acetate extract possessed free-radical scavenging and antimicrobial activities, which are desirable properties of a novel discovery for pharmaceutical and industrial applications. | en_US |
| dc.identifier.uri | http://hdl.handle.net/123456789/7794 | |
| dc.language.iso | en | en_US |
| dc.publisher | Universiti Sains Malaysia | en_US |
| dc.subject | The potency of eighty actinomycetes, isolated | en_US |
| dc.subject | mangrove sediment in states Perak and Kedah | en_US |
| dc.title | Isolation And Characterization Of Streptomyces sp. Isolated From Unexplored Estuarin Mangrove Sediments For Antioxidants Activities | en_US |
| dc.type | Thesis | en_US |
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