Mengenalpasti fenotip apolipoprorein E dengan menggunakan teknik fokus isoelektrik bagi kes-kes hiperlipidemia di Kelantan
Loading...
Date
1999
Authors
Rashid, Faridah Abdul
Mansur, Mazidah Ahmad
Nordin, Hasenan
Journal Title
Journal ISSN
Volume Title
Publisher
Kampus Kesihatan, Universiti Sains Malaysia
Abstract
Patients plasma samples (n=250) were screened for cholesterol and triglyceride content by enzymatic analyses performed on an automated chemistry analyzer using standard kits. Lipoprotein electrophoresis was performed on thin slab or pre-casted tube gels.Unrelated plasma were selected (n=24) and studied using both gel methods. One subject with a possible apolipoprotein E-2/2 phenotype was detected by the slab gel method. Another 11 subjects with unusual lipoprotein banding patterns and possible altered apolipoprotein E phenotypes were detected by the tube gel method. VLDL were isolated by a micromethod developed on an ultracentrifuge. Apolipoprotein E phenotype was confirmed by flat bed isoelectrtc focussing (IEF) of delipidated VLDL proteins on commercial and homemade IEF gels.
Description
Keywords
Cholesterol , Triglyceride , Lipoprotein electrophoresis