POTENTIAL OF Stichopus horrens AND Stichopus vastus EXTRACTS AS ANTICANCER AND WOUND HEALING AGENTS: EFFECTS ON BREAST CANCER AND FIBROBLAST CELL LINES
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Date
2014-08
Authors
AZEMI, NURUL ADILA
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Abstract
The commercial exploitation of sea cucumbers biomass due to healthy lifestyle are gaining much recognition among consumers, pharmaceutical industries and researchers. In peninsular Malaysia, the medicinal uses of sea cucumbers locally known as ‘gamat’ (Sea cucumbers of the Stichopodidae family), have been exploited. However there is still lacking of scientific research published regarding Stichopus horrens and Stichopus vastus biomass role especially for medicinal porposes, and whether this invertebrate actually possess bioactive compounds. In this study, Stichopus horrens and Stichopus vastus have been extracted using phosphate buffer saline and chloroform: methanol solvent. The ability of different crude extract obtained to induce cell death on MCF-7 and MDA-MB-231 cell lines was determined using MTS assay. Stichopus vastus PBS extract was found to be most cytotoxic on MCF-7 cells with IC50 value (65.14±5.59μg/ml) compare to MDA MB231 (73.09±7.32μg/ml). We also found that the Stichopus vastus PBS extract is cytoselective towards cancerous cells and does not inhibit the proliferation on MCF 10A at concentration less than 200μg/ml. The Stichopus vastus PBS extract induced the cell death via apoptosis was demonstrated by binding of phosphatidylserine on the conjugated cell membrane. However for Stichopus horrens extracts, the analysis was focused on others bioactive potential because the MTS assay
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results did not show potential effect on inhibit the proliferation of the cancer cells, MCF-7 and MDA MB 231 cells. The Stichopus horrens PBS and methanol extract were found to have good potential in wound healing process by accelerating the wound closure at certain concentration. Stichopus horrens methanol extract also helps to reduce the invasion of MDA MB 231 cells at concentration 50ug/ml. Based on our study, Stichopus vastus has potent anticancer activities and could therefore, potentially be a source for a pharmacogical active product suitable for development as chemotherapeutic agent and S. horrens may be able to play a significant role in prevent the invasion of the breast cancer cells and induce the wound healing process.
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POTENTIAL OF Stichopus horrens AND Stichopus vastus EXTRACTS