Total Sulfated Glycosaminoglycan (Gag) From Malaysian Sea Cucumbers Stichopus Hermanni And Stichopus Vastus And Its Effects On Wound Healing In Rats
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Date
2011-06
Authors
Masre, Siti Fathiah
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Publisher
Universiti Sains Malaysia
Abstract
Sea cucumbers have long been exploited as a source of medicinal compounds due to the presence of sulfated glycosaminoglycans (GAGs). The aim of this study was to investigate the occurrence of total sulfated GAG from the integument body wall, the visceral internal organs and the coelomic fluid of Malaysian sea cucumbers Stichopus hermanni and Stichopus vastus and evaluate the effect of total sulfated GAG on wound healing in rats using macroscopic and microscopic evaluations.
In both species, the integument body wall was the highest source of total, O- and N-sulfated GAGs followed by the visceral internal organs and the coelomic fluid. There was more O-sulfated GAGs compared to N-sulfated GAGs for percentage (%) division in both species. In the full-thickness excisional wound model using 47 female Sprague-dawley rats, 20 μl of 1 μg/ml concentration of total sulfated GAG from each anatomical part of each sea cucumber species were applied to the wound area (6 mm diameter) from Day0 to Day12, while phosphate buffered saline (PBS) was applied to control group. The progress of healing was assessed through macroscopic examination and analysis of epithelization, inflammatory cells, fibroblasts proliferation, new vessels formation and collagen fibers organisation using light microscope (LM), transmission electron microscope (TEM) and scanning electron microscope (SEM).
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Keywords
Sea cucumbers a source of medicinal compounds , due to the presence of sulfated glycosaminoglycans