Kajian Kesan Ekstrak Etanol Andrographis Paniculata Dan Andrografolida, Suatu Sebatian Bioaktifnya, Terhadap Aktiviti Enzim Metabolisme Fa Sa Ii Ugtdangst
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Date
2008-10
Authors
Ahmad, Norzainah
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Abstract
Andrographis paniculata (Hempedu bumi), from Acanthaceae family is
a traditional medicinal plant for diseases of malaria, inflammatory respiratory,
asthma, bronchitis, dysentery and many others. The effects of ethanolic
extracts of Andrographis paniculata (AP) (5, 50 and 500 IJg/mg) on UOPglucuronosyltransferase
(UGT) enzymes from microsome and glutathione-Stransferase
(GST) enzymes from post mitochondria of liver of male Sprague
Dawley (SO) rats were examined under in vitro conditions. The effects of
Andrographolide (AND) (concentrations of 5 and 50 IJg/mg), an important
bioactive compound of AP on UGT and GST enzymes were also studied in
vitro. In vivo studies involved the effect of AP extracts on normal male SO rats
(142---X 21 g) which received AP ethanol extracts (75 mg/kg/day) for 15
consecutive days and compared with control. UGT and GST enzyme activities
were studied with substrates p-nitrophenol and 1-chloro-2, 4 dinitrobenzene
(CONB) respectively, using spectrometric method. Results were compared
with control by using t test with P<0.05 as significant value. In vitro studies
showed that AP at concentration 5 IJg/mg significantly increased the UGT
enzyme activities but there was no effect on GST. AP at 50 IJg/mg showed no
effect on UGT and GST activities, however AP at 500 ~g/mg significantly
decreased the UGT and GST activities. In vitro study on AND concentrations
at 5 and 50 ~g/mg showed no changes on UGT and GST activities compared
to controls. In vivo study showed that the activity of UGT and GST enzymes
from rats treated with extract of AP 75 mg/kg/day for 15 days decreased
significantly (P<0.05) than controls. In conclusion, in vitro studies showed that
the extracts of AP at various doses had different effects on the activities of
UGT and GST enzymes. In vivo studies showed that AP given for 15 days
decreased significantly the enzyme activities of UGT and GST. AND at
concentrations of 5 and 50 ~g/mg had no effects on UGT and GST activities
(in vitro).
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Keywords
Andrographis paniculata (Hempedu bumi), from Acanthaceae family is a traditional medicinal plant , for diseases of malaria, inflammatory respiratory, asthma, bronchitis, dysentery and many others.