Morphological And Molecular Identification Of Aspergillus Species Isolated From Corn Feed In Penang
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Date
2015-01
Authors
ZULKIFLI, NOOR ATIQAH
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Abstract
Aspergillus is among the most prevalent fungus contaminating stored grains and feed including corn feed. The study of Aspergillus in corn feed is important to ensure the safety of corn feed used as livestock feed. Identification of Aspergillus can give indication of mycotoxin being produced, as different species produced different types of mycotoxin. In this study, morphological and molecular identification methods were used to identify Aspergillus from corn feed collected from sundry shops around Penang. Based on morphological characteristics, 202 isolates were tentatively identified as A. niger Groups I and II (116), A. flavus (32), A. oryzae (14), A. fumigatus (23), A. clavatus (2), and A. terreus (15). For molecular identification, DNA sequencing of ITS region and β-tubulin gene were used, and eight species were identified as A. niger (48), A. tubingensis (68), A. flavus (12), A. oryzae (5), A. fumigatus (11), A. clavatus (2), and A. terreus (7). Based on molecular identification, the representatives isolates of morphologically identified species were confirmed, with most isolates of Groups I were confirmed as A. niger and most isolates of Group II were confirmed as A. tubingensis. Phylogenetic analysis based on Neighbour Joining (NJ) and Maximum Likelihood (ML) methods with 1000 bootstrap values using individual and combined ITS region and β-tubulin sequences showed that the isolates from the same species were clustered in the same clade except A. flavus and A. oryzae isolates which were separated within the same main
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clade. The present study indicated that corn feed from sundry shops in Penang were contaminated with different types of Aspergillus species, and among the species were well known mycotoxin producers such as A. niger and A. flavus.
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Morphological And Molecular Identification Of Aspergillus Species Isolated , From Corn Feed In Penang