Development Of Method For Simultaneous Detection Of Dibutyl Phthalate And Oleamide In Stingless Bee Honey Harvested From Plastic Cups By Solvent Terminated Dispersive Liquid-Liquid Microextraction And Hplc

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dc.contributor.authorKoo, Yee Ping
dc.date.accessioned2016-09-09T08:18:50Z
dc.date.available2016-09-09T08:18:50Z
dc.date.issued2015
dc.description.abstractRecent increased demand for stingless bee honey in Malaysia have stimulated alternative methods to increase honey yields by using plastic cups as artificial honey pots within the beehives. However, honey’s acidity may lead to possible migration of plastic additives, where prolonged consumption may affect body’s physiological responses. This study was carried out to determine the presence of dibutyl phthalate (DBP) and oleamide in stingless bee honey collected from plastic cups. A rapid method for simultaneous extraction and detection of both analytes by Solvent Terminated Dispersive Liquid-Liquid Microextraction (ST-DLLME) coupled with High Performance Liquid Chromatography (HPLC) was developed. ST-DLLME parameters such as type of extraction solvent and disperser solvent were optimized. HPLC parameters optimized to produce good compound separation were Zorbax Eclipse XDB-C18 column, mobile phase acetonitrile: methanol (70:30) and detector wavelength 202nm with total runtime 10 minutes. Good linearity was observed for DBP ranged between 0.5-50 μg/g; and oleamide ranged between 10-250 μg/g. Limits of detection for DBP was 0.15μg/g; while oleamide was 4μg/g. Limits of quantitation for DBP and oleamide were 0.5μg/g and 11μg/g respectively. Spiked recovery for DBP was 61.6%, oleamide was 74.7%. The relative standard deviation for DBP and oleamide were 7.3% and 8.9% respectively (n=3). This developed method presents a sensitive and rapid detection procedure for both compounds, as it is able to detect concentrations below the specific migration limits determined by EU Commission 10/2011 for food contact materials. Both DBP and oleamide were not detected in all the honey samples collected.en_US
dc.identifier.urihttp://hdl.handle.net/123456789/2505
dc.language.isoenen_US
dc.publisherUniversiti Sains Malaysiaen_US
dc.subjectStingless bee honeyen_US
dc.titleDevelopment Of Method For Simultaneous Detection Of Dibutyl Phthalate And Oleamide In Stingless Bee Honey Harvested From Plastic Cups By Solvent Terminated Dispersive Liquid-Liquid Microextraction And Hplcen_US
dc.typeThesisen_US
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