Design And Evaluation Of Artificial Catalysts That Possess Enzyme-Like Amidolytic Catalysis
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Date
2017-11
Authors
Wong, Yoke Ming
Journal Title
Journal ISSN
Volume Title
Publisher
Universiti Sains Malaysia
Abstract
In the present study, with the main goal of mimicking the amidase activity of protease,artificial polymer catalyst and peptide catalyst were designed and synthesized followed by various evaluations to investigate the validity of the design to possess enzyme-like amidolytic catalysis. Poly(N-isopropylacrylamide) microgel(NMG) has been developed by adding various functional groups to control surface charges, hydrophobicity, pKaand protein adsorption capacitybut seldom for enzyme mimics. Here, NMGsanchored with three types of functional groups were developed and studied aspolymercatalyststo hydrolyze amide bonds under evaluated mild conditions. Various evaluationstrategies were testedfor efficient hydrolysis activity on a p-nitroaniline-based substrate by using a colorimetric assay.
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Keywords
Design And Evaluation , Artificial Catalysts , Possess Enzyme-Like Amidolytic Catalysis