Bioactivity Determination Of Cassia Surattensis Seed Extract
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Date
2015-09
Authors
Uthaya Kumar, U. Seeta
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Abstract
Cassia surattensis is a flowering plant that has been traditionally used in many countries for food and medicinal use. This study sought to determine the fingerprint profile, antioxidant activities, hepatoprotective effect, cytotoxicity effect on Vero cells and genoprotective activity using the Comet assay. The result of the HPTLC study revealed that the C. surattensis seed has 10 major different chemical component peaks and the heavy metal analysis showed that the seed was safe from high heavy metal contamination. The methanolic seed extract showed good antioxidant activities in 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity, nitric oxide radical scavenging activity and reducing power. C. surattensis seed extract contained total phenolic content of 100.99 mg GAE/g dry weight and there was a positive correlation between total phenolic content and the antioxidant activities of the seed extract. C. surattensis seed extract significantly reduced the elevated levels of serum liver enzymes and relative liver weight in paracetamol-induced liver hepatotoxicity mice. The histopathological examination verified the hepatoprotective effect of C. surattensis seed extract against the paracetamol-induced liver hepatotoxicity. In vitro cytotoxicity test demonstrated that C. surattensis seed extract was non cytotoxic against the Vero cells. C. surattensis seed extract also exhibited strong inhibitory effects against H2O2 - mediated DNA damage in Vero cells demonstrated that seed extract has an efficient antigenotoxic property. In conclusion, C. surattensis seed extract possessed antioxidant, hepatoprotective and genoprotective properties, which is probably due to the presence of phenolic content.