Uv-Visible Metabolomics Approach For The Determination Of Selected Adulterants In Claimed Premixed Coffee.
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Date
2015
Authors
Azizo Rahman, Muhammad Zul Fayyadh
Journal Title
Journal ISSN
Volume Title
Publisher
Universiti Sains Malaysia
Abstract
Adulterated premixed coffees have becoming an issue in Malaysia lately and has caught the eye of authorities due to death reports related to these products. The major cause of this issue reported that these premixed coffee passed the food inspection test and eventually released to the market for public. The coffee was claimed to be spiked with several sexual enhancers like sildenafil, tadalafil and vardenafill which are common drugs for treating erectile dysfunction. Metabolomics approach using UV-Vis spectroscopy was developed to detect the selected sexual enhancer drugs in the coffee commercial by employing SIMCA-P software version 13.0. Seven brands of coffee samples were purchased from local supermarket, 30 sachet each was tested totaling to 210 sample. Each sample were named H, J, G, W, N, T and K respectively. Three multivariate models were generated namely PCA, OPLS-DA and PLS-DA. OPLS-DA was chosen as the best model for overall results as it has minimal discriminate. Sildenafil, tadalafil and vardenafil were detected sample in H, vardenafil in brand J and none in sample G, W, N, T and K. The UV-Vis spectroscopy method had proven to be reliable and efficient in the determination of the selected drugs, as well as in saving time and cost.
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Keywords
Coffee