Phytochemical Analysis, Anticancer And Antiangiogenic Activities Of Barringtonia Racemosa Extracts And Its Fractions
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Date
2017-07
Authors
Amran, Norliyana
Journal Title
Journal ISSN
Volume Title
Publisher
Universiti Sains Malaysia
Abstract
Barringtonia racemosa Roxb. (Lecythidaceae) or locally known as putat sungai have been used in the treatment of cough, asthma, ulcer and diarrhea as traditional remedy. This study investigated anticancer activity of B. racemosa fruit extract, seed extract and all its fractions, in vitro. The crude extract and its potent bioactive fraction as anticancer agent were also been investigated for their antiangiogenic and antioxidant activity. The extraction of fruits and seed were carried out by maceration method and further separated into fractions by solvent-solvent partitioning starting from n-hexane, chloroform, ethyl acetate and n-butanol. The anticancer activity was investigated by in vitro cytotoxic on human breast cancer cell lines (MCF-7) and human colorectal cancer (HCT-116) by MTT assay. DNA fragmentation assay was used to identify the apoptosis mechanism of cell cancer death. The ex vivo antiangiogenic properties of potent bioactive anticancer fractions were determined using rat aorta ring assay. The antioxidant activities were assayed using 1, 1- diphenyl-2- picrylhydrazyl (DPPH), β-carotene linoleic acid bleaching and reducing power. The results demonstrated that B. racemosa n-butanol fraction exhibited an active cytotoxic effect on MCF-7 cell line (IC50 11.12 ± 1.05 μg/mL). The antiangiogenic activity of B. racemosa n-butanol fraction also showed 50 % inhibition of microvessels outgrowth in rat aortic rings at 34.55 ± 1.34 μg/mL. In antioxidant assay, the n-butanol fraction showed overall moderate activity among all assay tested. The chemical constituents present in the most bioactive faction, n-
butanol was detected by LCMS method.
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Keywords
Anticancer and antiangiogenic activities , of Barringtonia racemosa extracts