DETERMINATION OF NITROFURAN DERIVATIVES IN FISH AND SHRIMP TISSUES USING HPLC
| dc.contributor.author | TAN, SIN EE | |
| dc.date.accessioned | 2016-01-12T03:48:12Z | |
| dc.date.available | 2016-01-12T03:48:12Z | |
| dc.date.issued | 2003-04 | |
| dc.description.abstract | A high performance liquid chromatographic (HPLC) method with UVNis detection has been developed for the simultaneous determination of the three nitrofuran derivatives, nitrofurazone (NFZ), nitrofurantoin (NIT) and furazolidone (FZD) in fish and shrimp tissues. The drugs were first extracted with ethanol, and the lipids were removed from the extract with hexane. The ethanol extract was evaporated by rotary evaporation, and the resultant drug residues were passed through SPE. Two types of commercial sorbent . materials based on octadesylsilyl (Sep-Pak CIS) and aminopropyl (E~~~lean AP) functional groups were used. Higher recoveries were obtained using the Extract-Clean AP and thus it was selected for further analyses. Analytes were chromatographed isocratically with an octadecylsilyl CI8 (ODS) column and a UV-Vis detector set at 365 nm and identified by comparing the retention times. The HPLC method utilized a mobile system that consists of water : MeOH : O.OIM sodium acetate solution (5 : 15 : 80, v/v). The limit of detection based on a signal-to-noise ratio (SIN) of 3 was estimated to be 20 ppb for NFZ, 25 ppb for NFl' and 30 ppb for FZD. The overall average recovery (mean ± %RSD) in nitrofuran fortified fish and-shrimp tissues at 50 ppb were 85'.61 ± 7.73 % and 89;93 ± 3.69 % for NFZ; 87.24 ± 2.31 % and 89.49 % ± 1.37 % for NFl'; and 88.58 % ± 4.22 % and 88.48 % ± 4.80 % for FZD. Seven samples (5 cultured fish and 2 shrimp tissues) that were obtained locally were analyzed but no nitrofuran was detected. | en_US |
| dc.identifier.uri | http://hdl.handle.net/123456789/1468 | |
| dc.subject | DETERMINATION | en_US |
| dc.subject | NITROFURAN | en_US |
| dc.title | DETERMINATION OF NITROFURAN DERIVATIVES IN FISH AND SHRIMP TISSUES USING HPLC | en_US |
| dc.type | Thesis | en_US |
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