Biomass Optimization And Carrier Formulation For Microbial Inoculant Burkholderia Sp. Usmb20
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Date
2015-06
Authors
KEYEO, FLORY
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Abstract
Microbial inoculant is a sustainable environment-friendly system whereby beneficial
microbes were applied towards plants subsequently promoting growth of host plants. The aim of
the study is to (1) optimize the biomass of microbial inoculant Burkholderia sp. USMB20 (2)
determine carrier properties and assess inoculant survivability in carriers and (3) study the
effectiveness of inoculant towards host plant Mucuna bracteata. Response surface method
(RSM) was utilized in obtaining the optimal biomass of inoculant in shake flask studies and
subsequent bioreactor production was conducted. The inoculant was subjected to survivability
test under different ratio of rice husk and kaolin combinations by determining its survivability,
moisture content and pH level. The final test was in confirming the effectiveness of inoculant
towards its application on the host leguminous plant, Mucuna bracteata. The results successfully
showed that, optimal conditions for growth of inoculant was established at temperature 30°C, pH
7.0, fructose concentration at 1% (v/v) and yeast extract concentration at 0.06% (v/v). The
inoculant biomass was enhanced from 3.8 g/l in standard medium to 10.2 g/l that showed a 168%
increase. In carrier suitability test, the properties of rice husk and the mixture of rice husk and
kaolin indicated its potential as a good carrier for the microbial inoculant. The pelletized
inoculants recorded higher survivability up to week 19. The effectiveness of inoculant was also
confirmed during plant growth assessments. The pelletized inoculants in mixture of rice husk and
kaolin at the ratio of 1:1 are the superior carrier formulation and showed the best effect on plant
growth in terms of its consistency in nodule numbers and plant dry biomass. Collectively, this
study has optimized the biomass of inoculants to 10.2 g/l, determined survivability of inoculant
in storage up to 9.25 x 10 cfu/g on week 19 and proven its effectiveness towards growth of
Mucuna bracteata.
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Keywords
Biomass Optimization And Carrier Formulation , For Microbial Inoculant Burkholderia Sp. Usmb20