Identification And Characterization Of Colletotrichum Spp. From Anthracnose Of Guava (Psidium Guajava) And Banana (Musa Spp.), And Gliocephalotrichum Spp. From Fruit Rot Of Rambutan (Nephelium Lappaceum)
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Date
2013-12
Authors
Mohd Anuar, Intan Sakinah
Journal Title
Journal ISSN
Volume Title
Publisher
Universiti Sains Malaysia
Abstract
Fruit crops are vulnerable to postharvest diseases causing severe losses and
among the most common postharvest diseases are anthracnose and fruit rot. Studies on
postharvest disease on three fruit crops, namely guava (Psidium guajava), banana
(Musa spp.) and rambutan (Nephelium lappaceum) were conducted. From guava and
banana, anthracnose disease was observed, and two species of Colletotrichum were
identified. Based on morphological characteristics of colony colours, conidia,
appressoria and presence or absence of setae, the two species of Colletotrichum were
identified as C. gloeosporioides (52 isolates) and C. musae (six isolates). For
confirmation of species and phylogenetic analysis, sequencing of Internal Transcribed
Spacer (ITS) regions and ß-tubulin gene were applied. However, based on BLAST
results, 55 isolates were identified as C. gloeosporioides and only three isolates were
identified as C. musae. Phylogenetic analysis using ITS regions and ß-tubulin gene
sequences based on individual and combine datasets using Neighbour-joining (NJ) and
Maximum likelihood (ML) methods showed that C. gloeosporioides and C. musae
were clearly separated into different clades.
Description
Keywords
Colletotrichum Spp. , Rambutan (Nephelium Lappaceum)