The Effects Of Aerosol-Based Cell Delivery Technique On The Regeneration And Repair Of Airway Epithelium
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Date
2017-08
Authors
Egi, Kardia
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Journal ISSN
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Publisher
Universiti Sains Malaysia
Abstract
Aerosol-based cell delivery is a potential therapeutic strategy to overcome the debilitating reparative capacity of trachea and lung following injury. The goal of this study was to determine the effect of aerosol-based airway epithelial cell (AEC) delivery on tracheal repair and regeneration, lung damage, and local inflammatory responses in the setting of acute lung injury (ALI) induced by tracheal brushing in rabbit. AECs were isolated from the trachea of New Zealand white rabbits. In vitro aerosol delivery was performed to assess the viability of the cells. Brushing-induced tracheal injury was performed in a rabbit model to develop ALI. One day following injury, exogenous BrdU-labelled AECs were aerosolized using the MicroSprayer® Aerosolizer into the injured airway. Assessment involving safety and toxicity were carried out to observe the animal responses towards cell treatment. Histopathological assessments of the injury in the trachea and lungs along with local and systemic inflammatory responses were quantitatively measured at one and five days after cell delivery. Two in vitro co-culture assays were performed to investigate the functional effect of AECs on tracheal regeneration and repair. Under aerosol pressure, AECs were able to maintain a high viability rate without affecting their morphological features and proliferative capability compared. Brushing-induced tracheal injury exfoliated tracheal epithelium layer and triggered alveolar damage-associated ALI. Following treatment, aerosol-based AEC delivery appeared to be a safe procedure and positively modulate tracheal epithelium repair and regeneration, reduce inflammation, and attenuate lung injury in the rabbit model of ALI. The key mechanisms underlying this positive impact is due to the secretory factors that were released by AEC. This study
provides cellular and molecular insights of aerosol-based cellular therapy to form a basis evidence for future therapy to treat lung injuries.
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Keywords
The effect of aerosol-based airway epithelial cell , on tracheal repair and regeneration, lung damage