Micropropagation Of Curcuma Aeruginosa And Curcuma Heyneana Using Aerated Culture System

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dc.contributor.author., Novianti Suwitosari
dc.date.accessioned2019-06-26T01:51:36Z
dc.date.available2019-06-26T01:51:36Z
dc.date.issued2012-06
dc.description.abstractCurcuma aeruginosa and C. heyneana aseptic rhizomatous bud explants were established with immersion in 70% (v/v) ethanol for 10 minutes followed by surface-sterilization with 20% (v/v) Clorox® for 20 minutes. This surface-sterilization technique enabled 73.7% and 83.3% of C. aeruginosa and C. heyneana bud explants became aseptic with 66.7% and 87.5% survived explants, respectively. All the aseptic explants when cultured on MS medium supplemented with 2.0 mg/l BA and 0.5 mg/l NAA produced 1.9 shoots per explant for C. aeruginosa and C. heyneana produced 2.1 shoots per explant after four weeks of culture. The shoot explants of C. aeruginosa subsequently produced more multiple shoots when cultured in liquid shoot proliferation medium, while the number of shoots produced from C. heyneana was found to be no different in the gelled or liquid medium. When the shoots were incised longitudinally into halves and cultured in the liquid medium, both species produced significantly (p≤0.05) more multiple shoot. Incised shoots of C. aeruginosa produced 4.5 shoots per explant while C. heyneana produced 4.4 shoots per explant after four weeks of culture. Shoot explants of both species could produce an approximately 250 - 300 shoots in a 1L aerated vessel after four weeks of culture. All the micro-shoots of both species formed roots after transferred onto MS basic medium. All the in vitro plantlets of C. aeruginosa and C. heyneana (100%) survived during the acclimatization process using soil mixture of top soil: sand: organic soil (1:1:1). After 90 days, C. aeruginosa plantlets produced an average 2.3 micro-rhizomes per plantlet and C. heyneana produced only one micro-rhizome per plantlet.en_US
dc.identifier.urihttp://hdl.handle.net/123456789/8391
dc.language.isoenen_US
dc.publisherUniversiti Sains Malaysiaen_US
dc.subjectMicropropagation of Curcuma aeruginosaen_US
dc.subjectusing aerated culture systemen_US
dc.titleMicropropagation Of Curcuma Aeruginosa And Curcuma Heyneana Using Aerated Culture Systemen_US
dc.typeThesisen_US
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