Selected Pharmacological Studies Of Cinnamomum Iners Standardized Leaves Methanolic Extract
Loading...
Date
2011-12
Authors
Mustaffa, Fazlina
Journal Title
Journal ISSN
Volume Title
Publisher
Universiti Sains Malaysia
Abstract
Cinnamomum iners standardized leaves methanolic extract (CSLE) was subjected to phytochemical screening, antioxidant, toxicity studies, analgesic and antimicrobial activity. Phytochemical screening of CSLE showed the presence of cardiac glycoside, flavanoid, polyphenol, saponin, tannin and terpenoid. These secondary metabolites might be contributed to the bioactivity of CSLE. The overall antioxidant research provides information that CSLE showed moderate antioxidant activity. This might be due to the high phenolic content (211.94 ± 13.04 mg GAE/g plant material) in CSLE. Our study also investigated the safety parameters (toxicity) of CSLE to provide information regarding long term usage of this plant as traditional herbs. The toxicity screening of CSLE was conducted using brine shrimp assay, acute toxicity screening and histopathologic study. An acute toxicity study was carried out using OECD (The organization of Economic Co-operation Development) guideline 423. CSLE is considered safe for consumption with high LC50 and LD50 values. The analgesic activity of CSLE was evaluated using formalin, hot plate and tail flick tests at doses of 100, 200 and 500 mg/kg in mice. CSLE showed significant activity (P<0.05) in the formalin model (late phase) on the rats at 200 and 500 mg/kg. However, CSLE did not show activity in the hot plate and tail flick test. The results obtained suggest that CSLE act peripherally to relieve pain. As for the antimicrobial activity, CSLE, its fractions (hexane, ethyl acetate, butanol and aqueous) and the isolated compound of CSLE was investigated using different antimicrobial assays. CSLE and fractions were subjected to disc diffusion test, minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) using different gram positive, gram negative and yeast. Subsequent work was focused on the identification of antimicrobial compound by the means of bioautography. Bioautography assay of fractions revealed one compound of ethyl acetate fraction (Rf of 0.77) inhibited MRSA (methicillin resistant Staphylococcus aureus). Interestingly the same compound was found to inhibit Escherichia coli.
Description
Keywords
Pharmacological studies of Cinnamomum iners , standardized leaves methanolic extract