The effects of "gamat" on nociceptive processing in the central nervous system
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Date
2010
Authors
Hayati, Asma
Journal Title
Journal ISSN
Volume Title
Publisher
Pusat Pengajian Sains Perubatan, Universiti Sains Malaysia
Abstract
The aims of this study were to determine the analgesic dose ofholothuria extracts and
compare the extract effects with dynastat, cyclooxygenase-2 inhibitor, on pain
behaviour in rats injected with formalin. In the first part of the study, eighteen
Sprague-Dawley male rats (220-300 gram) were allocated into three different groups;
saline, holothuria 2 mglkg, and holothuria 4mg/kg. In the second part of the
investigation, twenty one Spra~e-Dawley male rats (220-300 gram) were given
either holothuria ( 4 mg/kg), saline or dynastat (20mglkg). The treatment was
administered intraperitoneally immediately after intraplantar injection of 0.05 ml
formalin (1 %). The rats' behaviour was recorded and behaviour data were analysed
using repeated measures analysis of variance (ANOV A) with post hoc Scheffe's test
and significance level was taken as 0.05. The study has demonstrated that the biphasic
response of the formalin test was markedly attenuated in the holothuria 4mglkg group,
signifYing inhibited pain behaviour. There was no significant difference between the
group receiving dynastat and salil)e from 5 to 50 minutes post formalin injection. At
55 and 60 minutes post formalin, the pain behaviour was significantly reduced for
both of the groups receiving holothuria (P< 0.01) and dynastat (P<0.05) when
compared to the saline group. Intraperitoneal administration of 4mglkg of holothuria
extracts has significantly suppressed the pain behaviour in formalin injected rats
compared to dynastat, a cyclooxygenase-2 inhibitor, in both phase I and 2 of formalin
test. Results from this investigation throw some light as to the possible use of gamat
extract as an analgesic.
Description
Keywords
Holothuria