Distribution Of Lachnum, Pteridocolous Fungi On Cyathea In Peninsular Malaysia

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dc.contributor.authorMohd Razikin, Muhammad Zulfa
dc.date.accessioned2017-02-02T01:44:20Z
dc.date.available2017-02-02T01:44:20Z
dc.date.issued2016-02
dc.description.abstractPteridocolous fungi are living and growing particularly on dead or decayed rachis of pteridophyte. Tree fern species in Malaysia are widely distributed in the lowland and adapted to the highland environment. In this study, samples of pteridocolous fungi were collected from Bukit Bendera, Bukit Larut, Cameron Highlands and Gunung Ledang. Based on the morphological characteristics, four Lachnum species namely L. oncospermatum, L. lanariceps, Lachnum sp. 1 and Lachnum sp. 2 were identified by distinguishing the shape of the apothecium, colour of resinous matter of the apothecium and the size of ascospores. In molecular study, 9 isolates were used for phylogenetic analyses. The DNA sequences of ITS-5.8S rDNA, the D1-D2 region of the large subunit rDNA, and combined data of ITS-5.8S and D1-D2 were analyzed. Phylogenetic trees were constructed by using Neighbour-Joining (NJ) and Maximum-Parsimony (MP) methods. The previous study of Lachnum species sequences with ITS and D1-D2 region were retrieved from the GenBank and included in the analysis for comparisons. L. oncospermatum, L. lanariceps and Lachnum sp. 1 were clearly separated into separate sub-clades. Lachnum sp. 2 grouped together with L. oncospermatum. The cultural characteristics of pteridocolous fungi were observed and recorded. The growth rate, the colony colour, and the pigmentation on various carbon sources in the medium were compared and recognized the substrate preference. Based on the utilization of carbon sources, the substrate preference for L. oncospermatum and Lachnum sp. 1 were sorbitol, sucrose, glucose and galactose as a carbon sources. The most suitable medium for the growth of L. lanariceps and Lachnum sp. 2 were on PDA. The present study indicated the identification and characterization of pteridocolous fungi in Peninsular Malaysia. This is the first molecular record of Lachnum species in Peninsular Malaysia.en_US
dc.identifier.urihttp://hdl.handle.net/123456789/3639
dc.language.isoenen_US
dc.publisherUniversiti Sains Malaysiaen_US
dc.subjectPteridocolous fungien_US
dc.titleDistribution Of Lachnum, Pteridocolous Fungi On Cyathea In Peninsular Malaysiaen_US
dc.typeThesisen_US
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