Effect Of Tualang Honey Treatment On Human Keloid And Normal Fibroblast And Its Relationship With TGF-B Expression
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Date
2011-10
Authors
Mohamad Shah, Nurul Syazana
Journal Title
Journal ISSN
Volume Title
Publisher
Universiti Sains Malaysia
Abstract
Keloid scar develops on the human skin after trauma, surgery or burn. Keloid grows beyond the boundaries of the wound site and is distinguishable from hypertrophic scar. Due to discomfort caused by keloid growth especially at exposed area, keloid excision might be needed. However, recurrence is common after surgical incision. Tualang honey is a local honey which is found on Tualang tree known as Koompassia excelsa found in Southeast Asia rainforests. Tualang honey is a multifloral honey which is produced by Apis dorsata or Asian rock bees that nested on the Tualang tree. There is no consensus and limited understanding in the keloid treatment using honey and therefore fundamental research is needed. The objectives of this study were to determine the degree of cytocompatibility of Tualang honey in vitro, to determine the chemical composition profile of Tualang honey, to investigate the effect of defined chemical components of Tualang honey on the proliferation of human skin fibroblasts in vitro and the effect on the expression of transforming growth factor-beta (TGF-β) in keloids after being treated with Tualang honey. The cultured keloid and normal fibroblasts were treated with several concentrations of Tualang honey ranging between 12.5% and 0.10% with 1:2 dilution followed by cytotoxicity testing using MTT and LDH assays. Volatile compounds of Tualang honey were identified based on five different organic solvents using Gas Chromatography-Mass Spectrometry (GC-MS) technique. Thirty five volatile compounds have been identified from the extracts of Tualang honey. Methanol extract of Tualang honey was used to treat fibroblasts and the cell proliferative effect was determined by MTS assay. Expression of TGF-βs in untreated and honey treated keloid fibroblasts were determined using TGF-β Immunoassay.
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Keywords
Effect of tualang honey treatment , human keloid and normal fibroblast