Bioavailability Studies Of Andrographolide In Rats
| dc.contributor.author | Chia, Lee Huang | |
| dc.date.accessioned | 2017-01-31T01:34:56Z | |
| dc.date.available | 2017-01-31T01:34:56Z | |
| dc.date.issued | 2016-06 | |
| dc.description.abstract | A simple, specific and sensitive high performance liquid chromatography (HPLC-UV) analytical method has been developed and validated for detection and quantification of andrographolide level in the plasma. The standard curve was linear from 31.3ng/mL to 2000ng/mL. The coefficient of variation (C.V.) values of this method was less than 12% for both within- and between-day validation. The accuracy for both within- and between-day validation ranged from 90.3% to 103.3% and 93.5% to 103.9% respectively. The absolute recovery for andrographolide was more than 67.3%. Andrographolide in working solution was stable up to 6 months. Nevertheless, it was only stable in plasma up to 2 hours, probably due to high protein-binding. Pure andrographolide was poorly soluble in water. Its aqueous solubility increased when physically mixed with polymer polyvinylpyrrolidone (PVP) K25. In vitro studies showed that the solubility of andrographolide increased when increased amount of PVP K25 was added. Andrographolide-PVP K25 complex was stable in pH1.2, 4.5 and 7.0 up to 8 hours, with more than 90% remaining undegraded. In vivo rat studies showed that pure andrographolide was barely detected in plasma although given at high dose of 50mg/kg. However, by mixing it with water-soluble polymer PVP K25, the bioavailability of andrographolide in rats increased significantly. Therefore, poor bioavailability of andrographolide is mainly due to its poor aqueous solubility. The poor bioavailability of andrographolide may also be to a lesser extent, due to first pass metabolism. | en_US |
| dc.identifier.uri | http://hdl.handle.net/123456789/3617 | |
| dc.subject | Andrographolide in working solution | en_US |
| dc.subject | was stable up to 6 months. | en_US |
| dc.title | Bioavailability Studies Of Andrographolide In Rats | en_US |
| dc.type | Thesis | en_US |
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