Protein identification of Anopheles Maculatus guts using matrix-assisted laser desorption/ionization – time of flight mass spectrometry
| dc.contributor.author | Liew, Hooi Ling | |
| dc.date.accessioned | 2014-11-03T02:19:33Z | |
| dc.date.available | 2014-11-03T02:19:33Z | |
| dc.date.issued | 2009 | |
| dc.description | Master | en_US |
| dc.description.abstract | Mosquito, a recognized vector, is linked to more human suffering than any other organisms, in which over one million people die from mosquito-borne diseases every year. According to the Center for Disease Control (Atlanta), malaria is a leading cause of premature mortality with around 5.3 million deaths annually, particularly in children under the age of five (WHO, 1996). The disease is most widespread in Africa, whereby a child is killed every 30 seconds (Florens et al., 2002; Knell, 1991). The mosquito of the Anopheles genus carries the malaria parasite, Plasmodium and the latter infects human and insect hosts alternately. This parasite has to complete a complex life cycle in vector mosquitoes, starting in the midgut lumen, crossing through the midgut epithelial barrier and lastly invading the salivary gland from where they can be transmitted from one host to another during blood feeding (Prévot et al., 1998; Shen et al., 1999; Zieler et al., 2001). Malaria incidence can be reduced by controlling parasite transmission by the mosquito. The Plasmodium develops from gamete to oocyst in the lumen and epithelium of the mosquito midgut (Abraham and Jacobs-Lorena, 2004; Knell, 1991). Trypsin, produced in the mosquito's digestive tract, probably activates parasite chitinase(s), facilitating the route of the parasite through the peritrophic matrix (PM) surrounding the parasite containing blood meal in the mosquito (Warr et al., 2007). Dimopoulos and coworkers (1998) found that early sporogonic stages of Plasmodium parasite alter the mosquito midgut immune response and also implied that this immune response molecule can be used to inhibit the development of the parasites (Tahar et al., 2002; Warr et al., 2007). However, most of the studies were done based on the analyses of mRNA production, moreover limited publication can be found concerning proteome analysis for mosquito midguts. | en_US |
| dc.identifier.uri | http://hdl.handle.net/123456789/265 | |
| dc.language.iso | en | en_US |
| dc.subject | Chemical science | en_US |
| dc.subject | Protein | en_US |
| dc.subject | Anopheles Maculatus | en_US |
| dc.subject | Flight mass spectrometry | en_US |
| dc.title | Protein identification of Anopheles Maculatus guts using matrix-assisted laser desorption/ionization – time of flight mass spectrometry | en_US |
| dc.type | Thesis | en_US |
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