DEVELOPMENT OF BACTERIAL CULTURE FOR ODOUR CONTROL
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Date
2011-08
Authors
PEl, CHIN SlAT
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Abstract
Volatile fatty acids particularly butyric acid is known as the major component of
odour, associated with livestock farming, agricultural activities and wastewater treatment
system. Thirteen butyric acid-degrading bacteria were isolated from the rivers and cow
farm in Malaysia. Screening with specific growth rates and ~utyric ~cid degradation rates
had selected four strains of highest values. These four bacteria were identified as
Cupriavidus sp. strain B3, Burkholderia sp. strain C2, Acinetobacter sp. strain C4 and
Cupriavidus sp. strain C5. Among the four, Acinetobacter sp. strain C4 showed the
highest values of specific growth rate at (0.321±0.002) h-\ and degradation rate at 54
ppm butyric acid/h. Further studies revealed that Acinetobacter sp. strain C4 was able to
perform butyric acid degradation at pH 6-9 and temperature of 25-40°C Additionally,
this bacterium was found degrading other volatile fatty acids such as acetic acid,
propionic acid, valerie acid and caproic acid, which were reported to deplete air quality
extensively. In the cells production course, cultivation medium was formulated with
1550 giL yeast extract. Acinetobacter sp. strain C4 was cultivated through exponential
feeding at 0.086 h-1 specific growth rate, and the cells concentration achieved was
(8.729±0.352) giL at 12 h of fed. A significant decline in growth thereafter was presumed
to be correlated with the generation of inhibitory metabolites. Hence, continuous culture
at different dilution rates (0.086, 0.20, 0.26, 0.35, OA3 h- 1
) were implemented to
eliminate substrate limitation and metabolites inhibition. The highest cells concentration,
(5.206±0.050) giL was obtained at 0.086 h- 1
, while the maximum cells productivity,
(0.926±0.034) giL/h was achieved at 0.20 h-1
• The fermentation studies showed that
continuous culture demonstrated the feasibility to achieve high cells productivity. The
produced cells were then immobilized in polyvinyl alcohol and incorporated in the
XVlll
biofilter development. The biofilter was constructed with a glass column of 24 x 2 em
(height x diameter) and packed with 24 g polyvinyl alcohol cubes containing 0.24 g dry
cells Acinetobacter sp. strain C4. Under aerobic condition, the propionic acid {452±2
ppm), butyric acid (1371±4 ppm) and valerie acid {1399±4 ppm) in cow farm wastewater
were removed at { 63 .4±2.2)%, ( 66.2±0.6)% and ( 61.2± 1.1 )%, respectively at 96 h.
Description
Keywords
DEVELOPMENT OF BACTERIAL CULTURE