Acute Oral Toxicity Classification Of Physalis minima Leaves Crude Water Extract Using The OECD TG423 On Female Sprague Dawley Rats

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Date
2015-08
Authors
Lokman, Nur Afiqah
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Publisher
Universiti Sains Malaysia
Abstract
The leaves of Physalis minima commonly known as “Daun Letup-letup” is a plant which is commonly used by the local community in Malaysia as a natural remedy in folk medicine and possess several bioactivities. P. minima exhibited a wide range of biological and pharmacological activities in a few laboratories studies and believed to have system of medicines for nervous disorder, epilepsy, liver antioxidant, antiinflammation, analgesic, antipyretic and as a helpful remedy in ulceration of the bladder. The toxicity of P. minima was important to identify of its safety. In order to assess the toxic class category of P. minima, OECD TG 423 was applied to determine P. minima toxicity involving limit dose of 2000 mg/kg by using healthy female Sprague Dawley albino rats (210-260 kg) were closely observed for 14 days. Six animals were used for each test. Acute toxicity study of P. minima extract was suspended in water and administered by oral gavage. Bodyweight for each rats was rrecorded at day 7 and day 14. All treated female SD rats survived the 14 day observation period and surviving animals were euthanized with carbon dioxide at day 14 and various tissues (brain, kidneys, liver, lung and spleen) collected, weighed and visually inspected for gross necropsy and histopathology was performed for further histopathological analysis. Based on UN-GHS decision logic, acute exposure of 2000 mg/kg of P. minima was safe in female rats without causing any adverse effects or mortality and was clasified under unclassified category.
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Keywords
Acute oral toxicity classification of , Physalis minima leaves Crude water extract
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