Understanding The Effects Of Garcinia Atroviridis Essential Oils In Combination With 2-Deoxy-D-Glucose On Pancreatic Cancer Cells (Panc 1)

Simple item page
dc.contributor.authorAziz, Fatin Athirah Abdul
dc.date.accessioned2022-08-16T02:35:14Z
dc.date.available2022-08-16T02:35:14Z
dc.date.issued2020-12
dc.description.abstractPancreatic cancer is among the highest lethal cancer in the world and current treatment and biomarker for this cancer is still limited. This study aimed to investigate the anti-proliferative effects of essential oils extracted from the leaves (EO-L) and stem bark (EO-B) of Garcinia atroviridis against human pancreatic cancer cell line, PANC- 1. The selective index (SI) values of each treatment was determined based on the ratio of IC50 in BEAS-2B cells in comparison to PANC-1 cells. In PANC-1 cells, IC50 and SI values of EO-L was 78 μg/ml and 1.23, respectively. EO-L at 100 μg/ml concentration had induced 73.97% the release of LDH after 24 h of incubation period. On the contrary, EO-B showed no pronounced anti-proliferative effect. Combination treatment between EO-L and 2-DG showed moderate synergism, with combination index (CI) values of 0.36 to 0.75. The effects of EO-L, 2-DG and EOL+2DG were further investigated on mitochondrial membrane potential (MMP), apoptosis, cell cycle and mRNA expression of selected regulatory molecules involved in apoptosis and cellular metabolism. EO-L had induced 57.9% MMP and 49.7% apoptosis in PANC-1 cells after 24 h of treatment. At 72 h of treatment, 2-DG and EOL+2DG caused 47.6% and 60.8% of MMP, respectively. Both 2-DG and EOL+2DG had caused PANC-1 cells to undergo apoptosis by 18.1% and 63.1%, respectively. Further, EO-L had induced 24.5% accumulation of cells at S phase after 48 h of treatment. At 24 h treatment, 2-DG and EOL+2DG had induced PANC-1 cells to be accumulated at G1 phase by 60.77% and 70.9%, respectively. EO-L had insignificant result in modulation of P53, HIF-1α, HK2 and CYP3A5 gene expression. 2-DG had significantly decreased the expression of HK2 by 0.45-fold difference and increased the expression of CYP3A5 by 44.7-fold difference relative to control cells. EOL+2DG had significantly increased the expression of P53 gene by 1.5-fold difference compared to control cells. Altogether, this study demonstrates the potential of EO-L from G. atroviridis in inhibiting the growth of PANC-1 cells through its actions by increasing the release of LDH, MMP, apoptosis and cell cycle arrest at S phase, despite no significant result in the mRNA expression of P53, HIFα, HK2 and CYP3A5. EO-L was found to synergize the actions of 2-DG in promoting cell death of PANC-1 cells.en_US
dc.identifier.urihttp://hdl.handle.net/123456789/15843
dc.language.isoenen_US
dc.publisherUniversiti Sains Malaysiaen_US
dc.subjectCanceren_US
dc.titleUnderstanding The Effects Of Garcinia Atroviridis Essential Oils In Combination With 2-Deoxy-D-Glucose On Pancreatic Cancer Cells (Panc 1)en_US
dc.typeThesisen_US
Files
License bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Institut Perubatan & Pergigian Termaju - Tesis