THE EFFECTS OF PSEUDOMONAS AERUGINOSA (USM=AR2) ON CRUDE OIL BIOMODIFICATION AND ITS POTENTIAL IN FACILITATING DISTILLATION
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Date
2009-06
Authors
MD NOH, NUR ASSHIFA
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Abstract
Pseudomonas aeruginosa (USM-AR2) culture was proven may facilitate
distillation process without reducing the quality of petroleum distillate obtained. This
strain yields 10 giL of biomass in shake flask batch culture experiments, an increase of
20-fold, after 120 hours cultivation, using 2% (v/v) inoculum, cultured in 100 mL
medium at an initial pH 5 at room temperature (27 °C) in the orbital shaker, agitated at
200 rpm. A suitable medium has been formulated, containing 5% (v/v) diesel, 0.6%
(w/v) yeast extract, 0.05% (w/v) MgS04 and 0.05% (v/v) Tween 80. Biosurfactant
activity (rhamnolipid) was detected by the orcinol assay, drop collapse test, surface
tension, and viscosity measurement of oil. Treatment of crude oil TK-57S and Angsi
with the improved culture of P. aeruginosa (USM-AR2) resulted in 2 to 3- fold decreases
of distillation times. No obvious difference was observed among treatment with cell
pellet, supernatant or the culture, suggesting that the biosurfactant is extracellular and cell
bound. Batch culture experiments in 1 L working volume bioreactor gave a biomass of
10 giL achieved after 72 hours incubation. Then, fixed volume pulse feeding strategy
with diesel oil as substrates resulted in 12 giL of cell biomass and 3.2 giL ofrhamnolipid
achieved in shorter time, after 42 hours cultivation.
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THE EFFECTS OF PSEUDOMONAS AERUGINOSA (USM=AR2) ON CRUDE OIL BIOMODIFICATION