Characterization and effect of Sn(IV) chlorin e6 dichloride trisodium salt in photodynamic therapy
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Date
2009
Authors
Al-Khaza’leh, Khaled Abdu Bdaiwi
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Abstract
This study was performed to investigate the effect of pH and serum on Sn(IV)
chlorin e6 dichloride trisodium salt photosensitizer and studying its effect in
photodynamic therapy.
This research consists of four stages. In the first stage the polarity of the
photosensitizer was examined and found to be more soluble in polar solvents with
high molar extinction coefficient. Acidification of the medium containing the
photosensitizer resulted in limited chemical changes in its pyrrole ring. There was no
significant effect of serum on the chemical structure of the photosensitizer.
In the second stage of this research the photobleaching of the photosensitizer
was affected by the presence of serum in the medium. The increasing of the
concentration of serum in the medium increases the photobleaching rate of Sn(IV)
chlorin e6 photosensitizer. The main factor responsible for the photobleaching was
found to be the singlet oxygen.
The thired stage of the research was to investigate the cellular uptake of Sn(IV)
chlorin e6 by cancer cell. It was found that the cellular drug uptake increases with the
decreasing of pH of the medium. On the other hand, the presence of serum in the
medium resulted in a reduction of the cellular drug uptake. Using HepG2 cancer cells
and CCD 18 CO normal cells it was found that the cellular drug uptake by cancer
cells is more than that in normal cell.
The final stage of this research was to study the effect of Sn(IV) chlorin e6 in
photodynamic therapy in vitro using MCF7, T47D and HepG2 cancer cell lines. It
was found that the photosensitizer has a lack of dark toxicity. Different incubation
times, different concentrations and different light doses were used to investigate the
effect of the photosensitizer in photodynamic therapy. The optimum incubation time
was about 6 hours while the optimum concentration was around 30 μg/ml and the
increasing of light dose increases the phototoxicity of the photosensitizer.
Description
PhD
Keywords
Science Physic , Dichloride trisodium salt , Photodynamic therapy