Characterization and effect of Sn(IV) chlorin e6 dichloride trisodium salt in photodynamic therapy

dc.contributor.author	Al-Khaza’leh, Khaled Abdu Bdaiwi
dc.date.accessioned	2014-11-03T02:20:25Z
dc.date.available	2014-11-03T02:20:25Z
dc.date.issued	2009
dc.description	PhD	en_US
dc.description.abstract	This study was performed to investigate the effect of pH and serum on Sn(IV) chlorin e6 dichloride trisodium salt photosensitizer and studying its effect in photodynamic therapy. This research consists of four stages. In the first stage the polarity of the photosensitizer was examined and found to be more soluble in polar solvents with high molar extinction coefficient. Acidification of the medium containing the photosensitizer resulted in limited chemical changes in its pyrrole ring. There was no significant effect of serum on the chemical structure of the photosensitizer. In the second stage of this research the photobleaching of the photosensitizer was affected by the presence of serum in the medium. The increasing of the concentration of serum in the medium increases the photobleaching rate of Sn(IV) chlorin e6 photosensitizer. The main factor responsible for the photobleaching was found to be the singlet oxygen. The thired stage of the research was to investigate the cellular uptake of Sn(IV) chlorin e6 by cancer cell. It was found that the cellular drug uptake increases with the decreasing of pH of the medium. On the other hand, the presence of serum in the medium resulted in a reduction of the cellular drug uptake. Using HepG2 cancer cells and CCD 18 CO normal cells it was found that the cellular drug uptake by cancer cells is more than that in normal cell. The final stage of this research was to study the effect of Sn(IV) chlorin e6 in photodynamic therapy in vitro using MCF7, T47D and HepG2 cancer cell lines. It was found that the photosensitizer has a lack of dark toxicity. Different incubation times, different concentrations and different light doses were used to investigate the effect of the photosensitizer in photodynamic therapy. The optimum incubation time was about 6 hours while the optimum concentration was around 30 μg/ml and the increasing of light dose increases the phototoxicity of the photosensitizer.	en_US
dc.identifier.uri	http://hdl.handle.net/123456789/274
dc.language.iso	en	en_US
dc.subject	Science Physic	en_US
dc.subject	Dichloride trisodium salt	en_US
dc.subject	Photodynamic therapy	en_US
dc.title	Characterization and effect of Sn(IV) chlorin e6 dichloride trisodium salt in photodynamic therapy	en_US
dc.type	Thesis	en_US