Detection And Quantification Of Amphetamine Type Stimulants And Methadone In Hair Using Liquid Chromatrography Triple Quadrupole (LC QQQ) Mass Spectrometry

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dc.contributor.authorAli Saad, Khadijah
dc.date.accessioned2019-06-24T01:10:48Z
dc.date.available2019-06-24T01:10:48Z
dc.date.issued2012-06
dc.description.abstractOver the last decade, analysis of hair for drugs of abuse has emerged as one important alternative to urine drug test. Hair analysis has been proposed as a more suitable marker of past chronic drug intake as drugs are present in the free form; hair provides a longer detection window making it difficult to cheat ; it is easy to collect and the sample is stable at room temperature for long period of time . Hair analysis is increasingly used in pre-employment and employment screening, in the forensic sciences, doping control and clinical diagnostics. Therefore, the aim of this study is to develop a sensitive and specific method for the detection and quantification of amphetamine type stimulants (ATS) and methadone in hair and urine, establish a correlation between the findings of urine and hair analysis in addicts and establish a correlation between the use of methadone and ATS in addicts. A method was developed and validated using liquid–liquid extraction followed by Liquid Chromatography Tandem Mass Spectrometry. The samples were extracted using liquid-liquid extraction following hydrolysis with NaOH in the presence of deuterated internal standards (d3-methadone,d5-methamphetamine, and d5-amphetamine). A bench top LC/MS/MS system based on a Liquid Chromatography Triple Quadrupole (LC QQQ) Mass Spectrometry with Electrospray ionization (ESI) was used. The optimization of MS/MS method was developed using the multiple reaction monitoring (MRM). For the purpose of confirmation, three diagnostic ions were monitored, the most abundant of which was chosen for the purpose of quantification and the second two were used for confirmation purposes.en_US
dc.identifier.urihttp://hdl.handle.net/123456789/8337
dc.language.isoenen_US
dc.publisherUniversiti Sains Malaysiaen_US
dc.subjectAnalysis of hair for drugs of abuse has emergeden_US
dc.subjectone important alternative to urine drug testen_US
dc.titleDetection And Quantification Of Amphetamine Type Stimulants And Methadone In Hair Using Liquid Chromatrography Triple Quadrupole (LC QQQ) Mass Spectrometryen_US
dc.typeThesisen_US
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