Isolation Of Antimigratory Chemical Constituents From Curcuma Caesia, Curcuma Aeruginosa Rhizomes And Dioscorea Bulbifera Tubers Using Human Breast Cancer Cell Lines
Loading...
Date
2021-04
Authors
Al-Amin, Md
Journal Title
Journal ISSN
Volume Title
Publisher
Universiti Sains Malaysia
Abstract
Curcuma caesia, Curcuma aeruginosa and Dioscorea bulbifera are used
traditionally for the treatment of human ailments including cancer. The present study
was carried out to search for antimigratory compounds from C. caesia, C. aeruginosa
rhizomes and D. bulbifera tubers using breast cancer cell lines. The chemical
constituents from C. caesia and C. aeruginosa rhizomes have been isolated through
fractionation. The extracts of C. caesia and C. aeruginosa rhizomes showed
concentration-dependent inhibition of MCF-7 and MDA-MB-231 cells. Further study
showed that hexane and chloroform fractions of C. caesia rhizomes and chloroform
fraction of C. aeruginosa rhizomes are the bioactive fractions that significantly inhibit
the viability of breast cancer cells. The chromatographic separation afforded
germacrone (1), zerumbone (2), furanodienone (3), curzerenone (4), curcumenol (5),
zederone (6), curcumenone (7) and dehydrocurdione (8) from hexane fraction and
curcuminol G (9), curcuzederone (10), (1S, 10S), (4S,5S)-germacrone-1(10), 4-
diepoxide (11), wenyujinin B (12), alismoxide (13), aerugidiol (14), zedoarolide B
(15), zedoalactone B (16) zedoarondiol (17) and isozedoarondiol (18) from
chloroform fraction of C. caesia rhizomes. The chromatographic separation afforded
germacrone (1), furanodienone (3), curzerenone (4), curcumenol (5) and zederone (6)
from chloroform fraction of C. aeruginosa. Cell viability assay of these isolated
compounds further revealed that compounds 1-4 and 10, and compounds 1, 3 and 4
are the bioactive compounds of C. caesia and C. aeruginosa, respectively. Germacrone (1) and curcuzederone (10) showed IC50 values of 246.3 and 227.2 μM against MDAMB-
231 cells, respectively. The activity of germacrone (1) and curcuzederone (10) on
the migration of MDA-MB-231 cells was also investigated and the results showed that
both compounds produced a significant concentration-dependent inhibitory activity.
These results indicate that germacrone (1) isolated from C. caesia and C. aeruginosa
rhizomes, and curcuzederone (10) isolated from C. aeruginosa rhizomes possess
antimigratory activities in breast cancer cell lines. The effect of the extract and
fractions of D. bulbifera tubers on cell migration was also investigated. The extract,
Fr.2 and Fr. 4, showed significant inhibition in a concentration-dependent manner on
the migration of MDA-MB-231 cells. Gelatin zymography assay showed that Fr.2 and
Fr.4 inhibited cell migration through the modulation of MMP-2 and MMP-9 enzymes.
The chromatographic separation of Frs. 2 and 4 yielded lusianthridin (19),
flavanthridin (20), 4,7-dihydroxy-2,3-dimethoxyphenanthrene (21), nudol (22)
catechin (23) and diosbulbin B (24). These results suggest that the antimigratory
activity of D. bulbifera tubers is possibly from compounds 19-24. In conclusion, the
findings presented within this thesis collectively support the traditional uses of C.
caesia, C. aeruginosa and D. bulbifera and provide an insight of the potential
therapeutic use of their chemical constituents to treat breast cancer.
Description
Keywords
Pharmacy