Optimization and biocompatibility of alumina foam coated scaffold for bone tissue engineering application
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Date
2016-05-01
Authors
Nor Suhaida Shahabudin
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Abstract
Alumina foam (AF) coated scaffold is one of synthetics biomaterials that has received much attention to overcome inertness properties for enhanced bone tissue bonding interaction. This research aims to produce AF coated scaffold with compressive strength higher than 2 MPa to 12 MPa, porosity higher than 70% to 99% and pores size are larger than 100 μm to 1000 μm which required in bone tissue engineering application. The significant factors in polymer foam replication (PFR) techniques are number of pores (ppi) of polyurethane (PU) foam, the composition ratio of solid loading to deionized water, percentage of binder and number of dipping process. The DOE analysis using 2k fractional factorial designs shows that the number of pore, composition ratio and number of dipping are most significant factors effect on the compressive strength of AF scaffold. According to the face-centered cube (FCC) design, the condition satisfied for human bone scaffold is prepared at 60/40 composition ratio, 20 ppi numbers of pores and three times of dipping process. The AF scaffold was further improved by coated with hydroxyapatite (HACAF) and hydroxyapatite-bentonite (HABCAF). The samples were evaluated by in-vitro analysis which is immersed in simulated body fluid (SBF) solution and cytotoxicity by MTS assay. The in-vitro analysis showed the surface of HACAF and HABCAF scaffold have precipitations of apatite layer and positive effect on cell growth.